Avila jerley, prabu dm 2015 purification, characterization and antioxidant properties of cphycocyanin from spirulina platensis scrutiny journal 2. Phycocyanin is a product of cultivation of spirulina platensis, a bluegreen microalga. In the present study an attempt has been made to develop a simple and efficient method for the extraction employing liquid nitrogen for cell disruption followed by enzymatic extraction of the phycocyanin. In the present study, we discussed the extraction, purification and characterization of cphycocyanin from a novel isolate oscillatoria quadripunctulata. Sivasankari, naganandhini and david ravindran department of biology, gandhigram rural institutedeemed university, dindigul, tamilnadu, india corresponding author abstract introduction spirulina is a bluegreen algae owing to the. Wo2014045177a1 method for extracting and stabilising. Production of antioxidant cphycocyanin using extraction process of. The purification steps include 1 extraction of phycocyanin from biomass, 2 ammonium sulfate precipitation of phycocyanin and dialysis, and 3 purification of phycocyanin by gel filtration and. Cphycocyanin cpc, a phycobiliprotein, is one of the key pigments of spirulina, a microalgae used in many countries as a dietary supplement. The influence of temperature and biomasssolvent ratio on phycocyanin extraction was evaluated using a full factorial design 2 2 plus star configuration with four replicates in the central points, which was a total of 12 treatment combinations box et al. Phycocyanin is a pigmentprotein complex from the lightharvesting phycobiliprotein family, along with allophycocyanin and phycoerythrin. Download fulltext pdf extraction of phycocyanin an important pharmaceutical phycobiliproteins from cyanobacteria article pdf available june 2014 with 1,064 reads. Spirulina and other bluegreen algae contain cphycocyanin, which acts as an accessory pigment when light energy is captured and transferred to chlorophyll a. Pdf methods for extraction, isolation and purification.
Optimization of cost effective method for extraction and fractionation. After ammonium sulphate precipitation the purity of phycocyanin was 1. Methods for extraction, isolation and purification of c. Select your pdf file from which you want to extract pages or drop the pdf into the file box. Extraction of astaxantine and phycocyanine from microalgae. Phycocyanin is a nontoxic, watersoluble pigment protein from microalgae that exhibits antioxidant, antiinflammatory, hepatoprotective, and neuroprotective effects. Four different wet methods were used for theextraction of phycocyanin and the following resultswere obtained. The short period of physical cphycocyanin extraction from arthrospira platensis. New research on extraction and purification of phycocyanin. Cphycocyanin extraction from spirulina platensis wet biomass. Purification involves multiple steps of crude extraction, including extraction with ammonium sulfate and separation on an ion exchange column, hydroxyapatite column or gel filtration chromatography. To validate the results reproducibility, each essay was done in triplicate. Application of an ultrafine shearing method for the extraction. Through repeatedly freezing and thawing alternatively in the presence of phosphate buffer combined with ultrasonic fragmentation,the phycocyanin was efficiently extracted from s.
Further extraction of phycocyanin pc showed that temperature greatly contributed to. The management of cyanobacteria and potential exposure to associated biotoxins requires the allocation of scarce resources across a range of freshwater resources within various jurisdictions. Recent developments in production and biotechnological. Therefore fresh wet biomass was suitable for phycocyanin extraction. Comparison of different extraction methods for phycocyanin extraction and yield from spirulina platensis s. Quantitative analysis of cphycocyanin from spirulina. Phycocyanin is a major light harvesting pigment of cyanobacteria, it has gained importance in many biotechnological applications in food sciences, therapy, immunodiagnostics, cosmetics and pharmacological processes. Phyocyanin extraction from microalgae spirulina platensis. The pharmacological actions, such as antiinflammation, antioxidation, antitumor, immunological enhancement, and hepatorenal protection of cphycocyanin cpc from spirulina. Compiled by a diverse team of experts, with experience in scientific and industrial fields, the comprehensive report for wastewater treatment using algae is the first report that provides indepth analysis and insights on this important field. Get a new document containing only the desired pages. Instead, phycobiliproteins aggregate to form clusters that adhere to the membrane called phycobilisomes.
Pdf the effects of temperature and frequencies in ultrasound. The extraction using ultrasonic bath in the presence of glass pearls in the biomass proved to be the most efficient method, 56% higher than using freezing and thawing the method most frequently used, and presented a extraction yield of 43. The highest content of phycocyanin is obtained on bean sprouts extract medium 8% vv with the addition of urea fertilizer 100 ppm with phycocyanin concentration of 257. Alternate method of phycocyanin extraction by will. The purpose of this project was to research the effectiveness of sonication technology to improve the efficiency and yields of phycocyanin production from the cyanobacterium spirulina platensis. Stable isolation of phycocyanin from spirulina platensis. Cell disruption is an important step during the extraction of cphycocyanin from. The aim of the present study is the isolation and characterization of thermostable phycocyanin from g. The total extraction yield of about 3% corresponds well to the. A simple method for efficient extraction and purification. From product literature, assuming phycocyanin sensor. The determination of phycocyanin purity is based on the absorbance ratio a 620 a 280, and the. Algal pigments have massive commercial value as natural colorants in nutraceutical, cosmetics, and pharmaceutical industries, besides their health benefits. Extraction and purification of phycocyanin, was completed in four major steps.
Alternative methods for analysis of cyanobacterial. Phycocyanin is an abundant proteinpigment complex in many photosynthetic microbes and the feedstock for several emerging products in the food and pharmaceutical industries. Method for efficient extraction of phycocyanin from. Five levels of each independent variable were chosen, the upper and lower limits of these being set in the range described in the. Cost effective and reliable methods for sample processing and analysis form the foundation of the protocol yielding reliable data from which to derive important decisions. Pdf phycocyanin is a pigmentprotein complex synthesized by bluegreen microalgae such. Crude cphycocyanin extracts from spirulina platensis, with and without cell debris, were purified in aqueous twophase systems atps varying the molar mass of the peg, at ph 6, more promising than at ph 7. Spirulina platensis has been cultivated recently in fed batch reactor to optimize the cell growth and product formation. The optimum method of extraction and purification of phycocyanin from spirulina platensis was improved in this paper. Extraction, isolation and purification of cphycocyanin biological value containing essential amino acids like phenylalanine and methionine.
The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer, from where it. Phycocyanin is a major phycobiliprotein produced by cyanobacteria, but only few strains for its efficient purification have been reported until now. A number of drying methods studied for the processing of spirulina oven dried. Drying methods were resulted in approximately 50% loss of phycocyanin. Phycocyanin not stable against ph, temperature, light and humidity 3,4. Extracting pages in pdf files does not affect the quality of your pdf. Molecular docking studies show that the phycocyanin dimer interacts with the enzyme. The tool extracts the pages so that the quality of your pdf remains exactly the same. The isolated phycocyanin from this process also showed highest purity of 3.
All phycobiliproteins are watersoluble, so they cannot exist within the membrane like carotenoids can. For run 2, in which 10% ethanol is added as a cosolvent, the extraction yield increased to about 3%. The total extract yield for run 1 extraction without cosolvent was of the order of 1. Extraction and purification of cphycocyanin from dry. The extraction, isolation and purification of cphycocyanin have been studied for many years, resulting in diverse methodologies with a range of yields and grades of purity. Pdf phycocyanin extraction in spirulina produced using. Physical extraction and extrusion entrapment of cphycocyanin from. Xifeng zhang 1, fenqin zhang 1, guanghong luo 2, shenghui yang 2, danxia wang 2. Evaluation of different extraction methods for phycocyanin. Phycocyanin has potential as a natural dye, but the effort required to maintain phycocyanin during storage. The purpose of this project was to research the effectiveness of. The htl biocrude oil obtained from the original spirulina feedstock and the residual after the cpc extraction were compared. The molecular weights of the phycocyanin isolated using the highpressure process and hexane separation method described in this study and those of the phycocyanin standard are shown in figure 1. N62dm, and the structure was solved to a resolution of 2.
Extraction and separation of phycocyanin from spirulina using aqueous twophase systems of ionic liquid and salt. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a pdf plugin installed and enabled in your browser. For the assays carried out with each molar mass, with distinct percentages of peg and salt, the concentrations mg ml1, purities od 620od 280 and cphycocyanin partition. Medical application of spirulina platensis derived c. Burkert cav, kalil sj 2007 optimization of phycocyanin extraction from spirulina platensis using. Efficacy of different extraction methods of phycocyanin. Purification and bioactivities of phycocyanin request pdf. The extraction, isolation and purification of c phycocyanin have been studied for many years, resulting in diverse methodologies with a range of yields and grades of. Comparison of different extraction methods for phycocyanin. Although a number of reports are available for the extraction and puri. Research article phycocyanin extraction from spirulina platensis and extract stability under various ph and temperature.
Phycocyanin extraction is carried out from wet or dry biomass. Pc, a natural blue dye, has been used in food, immunodiagnostics and analytical reagents. A valuable and practical working handbook containing introductory and specialist content that tackles a major and growing field of environmental, microbiological and ecotoxicological monitoring and. Handbook of cyanobacterial monitoring and cyanotoxin. B 12 cyanocobalamin, b 6 pyridoxine, b 1 thiamine and b 2 riboflavin, as well as beta. Organization instrument excitation nm emission nm massdep turner designs databank datalogger with cyclops 7 probe phycocyanin sensor 590 650 us epa ysi multisensor probesondes 565605. The residual biomass after cpc extraction was converted into biocrude oil via hydrothermal liquefaction htl. First, the biomass underwent different treatments for cell disruption, including drying, freezing and milling at different levels, and the extraction was then. Phycocyanin is widely used in medical treatment as a cancer inhibitor and as a natural dye for cosmetics and foods. In spirulina species, phycocyanin pigments increase from 12% to 22% on increasing nitrogen levels. Pdf phycocyanin extraction from spirulina platensis and extract.
In the present study, intact hexameric phycocyanin was isolated and crystallized from the cyanobacterium leptolyngbya sp. Of several different methods tried for the extraction of phycocyanin pc from the cyanobacterium spirulina, treatment with 1800 units ml lysozyme and 100 ppm linear alkyl benzene sulfonate afforded good pc yield with high specific content, resulting in. Extraction and separation of phycocyanin from spirulina. Optimization of phycocyanin extraction from spirulina. Quantitative analysis of cphycocyanin from spirulina pacifica low temperature method background. Said method is characterised in that same includes a step of macerating the material or materials in glycerol or a waterglycerol mixture. So far, phycocyanins are isolated from algae in all studies. Extraction and characterisation of analytical grade cphycocyanin. Algae phycocyanin,production, uses and market oilgae. Isolation and characterization of thermostable phycocyanin. Using realtime monitoring and phycocyanin probes to track.
Along with the development of marine biological pharmaceutical research, higheffective and lowtoxic drugs and functional foods isolated from marine organisms have become a new field of pharmacy and bromatology. By considering various significant biological applications of cpc, the aim of this study was extraction and purification of cphycocyanin from the dry spirulina powder using various. Ultrasonicassisted extraction of cphycocyanin showed high efficiency. The extraction of phycobilliproteins involves cell rupture and release of these proteins from within the cell. The crude extract was precipitated by 50% saturation ammonium sulfate, and purified by hydrophobic interaction chromatography using phenyl sepharose 6 ff. Phycocyanin extraction, spirulina platensis, bluegreen algae a wide range of phycocyanin extraction methods were studied for the processing of spirulina biomass.
An extensive range of pigments including phycobiliproteins are present in algae. Extraction and purification of cphycocyanin from spirulina platensis ccc540. Avila jerley, prabu dm 2015 purification, characterization and antioxidant properties of c phycocyanin from spirulina platensis scrutiny journal 2. Extraction and purification of c phycocyanin from spirulina platensis ccc540. The invention relates to a method for extracting and stabilising phycocyanin from at least one material containing same. In addition to these health benefits, this pigment has been used in dietary nutritional supplements and natural colorant applications in the food, nutraceutical, cosmetic, and. To increase the extraction yield, intensification techniques may be used as pretreatment or simultaneously with the extraction. A largescale preparation method of high purity cphycocyanin.1500 1307 1073 1053 1201 974 901 863 481 301 345 57 306 620 941 1240 314 664 1116 1230 178 628 1127 1382 568 768 1161 318 172 806 492 345 1257 994 556 1168 907 1420 781 1060 1264